Identification of a cross-reacting, monoclonal anti-human CD233 antibody for identification and sorting of rhesus macaque erythrocytes

Cytometry A. 2012 Feb;81(2):165-8. doi: 10.1002/cyto.a.22005. Epub 2011 Dec 13.

Abstract

Erythroid biology research involving rhesus macaques has been applied to several topics including malaria, hemoglobinopathy and gene therapy research. However, analyses of the rhesus red blood cells are limited by the inability to identify and sort those cells in research blood samples using flow cytometry. Here it is reported that the BRIC 6 hybridoma clone raised to the human erythroid surface molecule (referred to as CD233, Band 3, AE1, or SLC4A1) produces cross-reactive and erythroid-specific antibodies for flow cytometric detection and sorting of rhesus macaque erythrocytes.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Anion Exchange Protein 1, Erythrocyte / chemistry
  • Anion Exchange Protein 1, Erythrocyte / immunology*
  • Antibodies, Monoclonal / immunology*
  • Cross Reactions / immunology*
  • Erythrocytes / cytology
  • Erythrocytes / immunology*
  • Flow Cytometry / methods*
  • Glycophorins / metabolism
  • Humans
  • Macaca mulatta / immunology*
  • Molecular Sequence Data

Substances

  • Anion Exchange Protein 1, Erythrocyte
  • Antibodies, Monoclonal
  • Glycophorins
  • SLC4A1 protein, human