Abstract
A multiplex methodology using two nuclease-resistant molecular beacons that target specific genomic regions of adenovirus 2 and echovirus 17 during simultaneous infection in A549 cells is presented. Using fluorescence microscopy, visualization of enteroviral and adenoviral replication was possible within 3 h postinfection.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Adenovirus Infections, Human / diagnosis*
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Adenovirus Infections, Human / virology
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Adenoviruses, Human / isolation & purification*
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Cell Line
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Echovirus Infections / diagnosis*
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Echovirus Infections / virology
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Enterovirus B, Human / isolation & purification*
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Humans
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In Situ Hybridization, Fluorescence / methods*
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Microscopy, Fluorescence
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Time Factors
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Virology / methods*
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Virus Replication