Abstract
The multiresistance gene cfr was identified for the first time in an Enterococcus faecalis isolate of animal origin. The 32,388-bp plasmid pEF-01, which carried the cfr gene, was sequenced completely. Three copies of the insertion sequence IS1216 were identified in pEF-01, and the detection of a cfr- and IS1216-containing amplicon by inverse PCR suggests that IS1216 may play a role in the dissemination of cfr by a recombination process.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Anti-Bacterial Agents / pharmacology*
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Bacterial Proteins / genetics*
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Cattle
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Cattle Diseases / microbiology*
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Chloramphenicol / pharmacology
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DNA Transposable Elements / genetics
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DNA, Bacterial / genetics*
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Drug Resistance, Multiple, Bacterial / genetics
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Enterococcus faecalis / drug effects
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Enterococcus faecalis / genetics*
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Enterococcus faecalis / isolation & purification
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Gene Dosage
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Gram-Positive Bacterial Infections / drug therapy
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Gram-Positive Bacterial Infections / microbiology
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Gram-Positive Bacterial Infections / veterinary*
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Methyltransferases / genetics*
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Mutagenesis, Insertional
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Plasmids
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Polymerase Chain Reaction
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Recombination, Genetic
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Thiamphenicol / analogs & derivatives
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Thiamphenicol / pharmacology
Substances
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Anti-Bacterial Agents
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Bacterial Proteins
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DNA Transposable Elements
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DNA, Bacterial
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Chloramphenicol
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florfenicol
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Methyltransferases
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Thiamphenicol