Objectives: To check TaqMan Realtime PCR in detecting genotypes of hepatitis C virus in Iran.
Methods: From July 2007 to April 2009, HCV genotyping was done on 52 patients who were referred to Research Centre for infectious Disease and Tropical Medicine, in Bou-Ali Hospital, Zahedan University of Medical Sciences. All these patients had proven hepatitis C infection.
Results: Out of 52 anti HCV positive samples, 28 (53.84%) had genotype 1, 2 cases (3.88%) had genotype 2, 12 (23.08%) had genotype 3 and 7 (13.4%) had genotype 4. Mixed infection with genotypes 1 and 3 was seen in 3 cases (5.77%).
Conclusion: TaqMan probes for detecting genotyping of HCV were successful in picking genotyping of HCV infection especially those with mixed genotypes.