Comparison of preserved vascular allografts using glycerol and University of Wisconsin solution in a goat carotid artery transplantation model

Eur Surg Res. 2012;48(2):64-72. doi: 10.1159/000334170. Epub 2011 Dec 30.

Abstract

Background: Prosthetic grafts have poor patency rates in peripheral arterial reconstructions. Glycerol (GL)-preserved grafts are an alternative. The aim of this study was to examine patency, graft morphology and function of GL-preserved allografts in a goat carotid artery animal model.

Methods: The first group (n = 7) underwent bilateral replacement of the carotid artery by a carotid allograft that was preserved in GL for 1 week. In the second group (n = 5), a carotid artery allograft that was preserved in University of Wisconsin solution (UW) for 48 h was used. In the third group (n = 5), the jugular vein (autologous vein, AU) was used. The follow-up was 3 months.

Results: One UW graft and 1 GL graft occluded in the first 24 h postoperatively. Three-month primary patency rates for GL, UW and AU grafts were 93, 100 and 80%, respectively (p = 0.39). Graft diameter was increased in UW allografts (p < 0.005), whereas GL allografts remained unchanged. After explantation, GL allografts demonstrated contraction and relaxation capacity and lower intimal thickness (p < 0.001).

Conclusion: GL preservation has proven to be a feasible method for arterial allograft transplantation in a large animal model with decreased intimal hyperplasia and renewed functional capability.

Publication types

  • Comparative Study

MeSH terms

  • Adenosine
  • Allopurinol
  • Angiography
  • Animals
  • Blood Flow Velocity
  • Carotid Arteries / physiology
  • Carotid Arteries / transplantation*
  • Carotid Arteries / ultrastructure
  • Feasibility Studies
  • Glutathione
  • Glycerol*
  • Goats
  • Insulin
  • Microscopy, Electron, Scanning
  • Organ Preservation
  • Organ Preservation Solutions*
  • Raffinose
  • Systole
  • Transplantation, Homologous
  • Vascular Patency*
  • Vasoconstriction

Substances

  • Insulin
  • Organ Preservation Solutions
  • University of Wisconsin-lactobionate solution
  • Allopurinol
  • Glutathione
  • Adenosine
  • Raffinose
  • Glycerol