Molecular mechanism underlying the cerebral effect of Gly-Pro-Glu tripeptide bound to L-dopa in a Parkinson's animal model

Amino Acids. 2012 Sep;43(3):1359-67. doi: 10.1007/s00726-011-1210-x. Epub 2012 Jan 5.

Abstract

Oxidative stress is a critical contributing factor to neurodegenerative disorders. Therefore, the inhibition of ROS formation, responsible for chronic detrimental neuroinflammation, is an important strategy for preventing the neurodegenerative disease and for neuroprotective therapy. Gly-Pro-Glu (GPE) is the N-terminal tripeptide of insulin-like growth factor-I, which is naturally cleaved in the plasma and brain tissues. GPE has neuroprotective effects since it crosses the blood-CSF and the functional CSF-brain barriers and binds to glial cells. It has been shown that GPE improves motor behaviour in rats after 6-OHDA lesion, although it does not rescue dopaminergic neurons. Thus, we hypothesized that the GPE therapeutic efficacy in a Parkinson model might be improved by combining GPE to L: -dopa. Here, we used an animal model that represents a progressive chronic Parkinson's disease (PD) model, characterized by high levels of oxidative stress and inflammation. We showed that the co-drug, in which L: -dopa is covalently linked to the GPE tripeptide, by down-regulating the expression of inflammatory genes, decreases the 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced inflammatory response and, by up-regulating tyrosine hydroxylase, reduces MPTP-induced neurotoxicity. Furthermore, by determining the nuclear translocation/activation of Nrf2 and NF-κB, we showed that systemic administration of the co-drug activates Nrf2-induced antioxidant response while suppressing NF-κB inflammatory pathway. Data suggest that the binding of L: -dopa to GPE tripeptide might represent a promising strategy to supply L: -dopa to parkinsonian patients.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analysis of Variance
  • Animals
  • Basal Ganglia / drug effects*
  • Basal Ganglia / metabolism
  • Basal Ganglia / pathology
  • CD11b Antigen / genetics
  • CD11b Antigen / metabolism
  • Disease Models, Animal
  • Dopamine / metabolism
  • Drug Evaluation, Preclinical
  • Gene Expression / drug effects
  • Glial Fibrillary Acidic Protein / genetics
  • Glial Fibrillary Acidic Protein / metabolism
  • Glutathione / metabolism
  • Heme Oxygenase-1 / metabolism
  • Levodopa / administration & dosage
  • Levodopa / analogs & derivatives*
  • Levodopa / chemical synthesis
  • Male
  • Mice
  • Mice, Inbred C57BL
  • NF-E2-Related Factor 2 / metabolism
  • NF-kappa B / metabolism
  • Neuroprotective Agents / administration & dosage*
  • Neuroprotective Agents / chemical synthesis
  • Nitric Oxide Synthase Type II / metabolism
  • Oligopeptides / administration & dosage*
  • Oligopeptides / chemical synthesis
  • Oxidative Stress
  • Parkinsonian Disorders / drug therapy*
  • Tumor Necrosis Factor-alpha / metabolism
  • Tyrosine 3-Monooxygenase / genetics
  • Tyrosine 3-Monooxygenase / metabolism

Substances

  • Ac-LD(Ac)2-Gly-Pro-Glu(OMe)-OMe
  • CD11b Antigen
  • Glial Fibrillary Acidic Protein
  • NF-E2-Related Factor 2
  • NF-kappa B
  • Neuroprotective Agents
  • Nfe2l2 protein, mouse
  • Oligopeptides
  • Tumor Necrosis Factor-alpha
  • Levodopa
  • Nitric Oxide Synthase Type II
  • Nos2 protein, mouse
  • Heme Oxygenase-1
  • Tyrosine 3-Monooxygenase
  • Glutathione
  • Dopamine