Endogenous digitalis-like factor as a stimulator of endothelin secretion from endothelial cells

Biochem Biophys Res Commun. 1990 Oct 15;172(1):178-83. doi: 10.1016/s0006-291x(05)80190-1.

Abstract

Effects of human urine-derived endogenous digitalis-like factor (EDLF) and ouabain on endothelin (ET) secretion were examined in cultured endothelial cells. ET was secreted in a linear fashion over 5 hours from bovine pulmonary artery endothelium into serum-free medium. EDLF stimulated ET secretion in a dose-dependent manner. In contrast, ouabain did not affect ET secretion at the concentration of 10(-9)-10(-5) M. These results indicate that human urine-derived EDLF is distinct from plant-derived ouabain and act as a stimulator of ET secretion by endothelial cells.

MeSH terms

  • Animals
  • Blood Proteins / isolation & purification
  • Blood Proteins / pharmacology*
  • Blood Proteins / urine
  • Cardenolides
  • Cattle
  • Cells, Cultured
  • Digoxin*
  • Endothelins / metabolism*
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology*
  • Humans
  • Kinetics
  • Ouabain / pharmacology*
  • Pulmonary Artery
  • Radioimmunoassay
  • Saponins*

Substances

  • Blood Proteins
  • Cardenolides
  • Endothelins
  • Saponins
  • digoxin-like factors
  • Ouabain
  • Digoxin