Magnetite-loaded fluorine-containing polymeric micelles for magnetic resonance imaging and drug delivery

Biomaterials. 2012 Apr;33(10):3013-24. doi: 10.1016/j.biomaterials.2011.12.042. Epub 2012 Jan 13.

Abstract

Magnetite (Fe(3)O(4)) - loaded polymer micelles (denoted as "magnetomicelles") are produced by self-assembly of fluorine-containing amphiphilic poly(HFMA-g-PEGMA) copolymers with oleic acid modified Fe(3)O(4) nanoparticles in an aqueous medium. The oleic acid modified Fe(3)O(4) nanoparticles form small clusters in the poly(HFMA-g-PEGMA) micelles with a mean diameter of 100 nm and the magnetomicelles show high stability in an aqueous medium due to the high hydrophobic fluorine segments in graft copolymers enhance the stability of the micelles. The magnetomicelles also show good cytocompatibility based on the MTT cytotoxicity assay and possess paramagnetic properties with saturation magnetization of 17.14 emu/g.Their good stability, cytocompatibility, and paramagnetic properties render the materials attractive in drug delivery and in vivo magnetic resonance imaging (MRI) applications. Controlled release of hydrophobic drug-5-fluorouracil is achieved from the magnetomicelles with a loading efficiency of 20.94 wt%. The magnetomicelles have transverse relaxivity rates (r(2)) of 134.27 mM(-1) s(-1) and exhibit high efficacy as a negative MRI agent in T(2)-weighted imaging. In vivo MRI studies demonstrate that the contrast between liver and spleen is enhanced by the magnetomicelles. These favorable properties suggest clinical use as nanocarriers in drug delivery applications and contrast agents in MRI.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Death / drug effects
  • Cell Survival / drug effects
  • Chromatography, Gel
  • Drug Delivery Systems / methods*
  • Fluorine / chemistry*
  • Fluorouracil / pharmacology
  • HeLa Cells
  • Humans
  • Hydrodynamics
  • Magnetic Resonance Imaging / methods*
  • Magnetic Resonance Spectroscopy
  • Magnetite Nanoparticles / chemistry*
  • Magnetite Nanoparticles / ultrastructure
  • Methacrylates / chemistry*
  • Mice
  • Micelles*
  • Organ Specificity / drug effects
  • Polyethylene Glycols / chemistry*
  • Spectroscopy, Fourier Transform Infrared
  • Staining and Labeling
  • Surface Tension / drug effects
  • Surface-Active Agents / chemistry
  • Thermogravimetry
  • X-Ray Diffraction

Substances

  • Magnetite Nanoparticles
  • Methacrylates
  • Micelles
  • Surface-Active Agents
  • polyethylene glycol methacrylate
  • Fluorine
  • Polyethylene Glycols
  • Fluorouracil