Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi

Stephanie Engel; Maren de Vries; Andreas Herrmann; Michael Veit

doi:10.1016/j.febslet.2012.01.002

Mutation of a raft-targeting signal in the transmembrane region retards transport of influenza virus hemagglutinin through the Golgi

FEBS Lett. 2012 Feb 3;586(3):277-82. doi: 10.1016/j.febslet.2012.01.002. Epub 2012 Jan 10.

Authors

Stephanie Engel¹, Maren de Vries, Andreas Herrmann, Michael Veit

Affiliation

¹ Immunology and Molecular Biology, Veterinary Medicine Faculty, Free University, Berlin, Germany.

PMID: 22245151
DOI: 10.1016/j.febslet.2012.01.002

Abstract

Inclusion of proteins into membrane-rafts favours interactions required for virus assembly but has also been proposed to facilitate vesicular transport of proteins. The hemagglutinin (HA) of influenza virus contains a raft-targeting sequence in the outer leaflet of its transmembrane region. We report that its mutation enhances co-localization of HA with a cis-Golgi marker and retards Golgi-localized processing, such as acquisition of Endo-H resistant carbohydrates and proteolytic cleavage. In contrast, trimerization of the molecule in the ER and transport to the apical membrane were not affected. The second signal for raft-targeting, S-acylation at cytoplasmic cysteines, did not retard HA transport.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Dogs
Exocytosis
Golgi Apparatus / metabolism*
Hemagglutinin Glycoproteins, Influenza Virus / chemistry*
Hemagglutinin Glycoproteins, Influenza Virus / genetics
Hemagglutinin Glycoproteins, Influenza Virus / metabolism*
Membrane Microdomains / metabolism*
Mutation*
Protein Sorting Signals / genetics*
Protein Transport

Substances

Hemagglutinin Glycoproteins, Influenza Virus
Protein Sorting Signals