Background: Combined resistance to quinolones and β-lactams is common in Enterobacteriaceae. The appearance in enterobacteria coding for metallo-β-lactamases and determinants of plasmid-mediated quinolone resistance are an emerging problem in our country.
Methods: The susceptibility was determined by E-test. The resistance genes were detected by PCR and the corresponding plasmids were characterised.
Results: This study describes 2 strains (1 Klebsiella oxytoca, 1 Klebsiella pneumoniae) carrying the genes qnrS2 and blaVIM-1 in a transferable plasmid of 70-Kb isolated in surveillance cultures at the University Hospital Virgen Macarena in Seville.
Conclusion: This is the first combination of qnrS2 and bla(VIM-1) on the same non-typeable plasmid isolated in our centre.
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