Abstract
A novel two-step multiple displacement amplification-PCR (MDA-PCR) assay for tuberculosis detection in 200 sputum specimens was evaluated. The MDA-PCR assay indicated a significant increase in sensitivity and specificity compared with those of standard PCR alone.
Publication types
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Comparative Study
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Evaluation Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Case-Control Studies
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DNA Transposable Elements / genetics*
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DNA, Bacterial / genetics*
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Humans
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Molecular Diagnostic Techniques
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Mycobacterium tuberculosis / genetics*
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Polymerase Chain Reaction / methods*
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Sensitivity and Specificity
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Sputum / microbiology
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Tuberculosis, Pulmonary / diagnosis*
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Tuberculosis, Pulmonary / microbiology
Substances
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DNA Transposable Elements
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DNA, Bacterial