Stylissamide X, a new proline-rich cyclic octapeptide as an inhibitor of cell migration, from an Indonesian marine sponge of Stylissa sp

Bioorg Med Chem Lett. 2012 Feb 15;22(4):1818-21. doi: 10.1016/j.bmcl.2011.10.023. Epub 2011 Oct 13.

Abstract

A new proline-rich cyclic octapeptide named stylissamide X (1) was isolated from an Indonesian marine sponge of Stylissa sp. as an inhibitor of cell migration from the guidance of wound-healing assay. The chemical structure of stylissamide X (1) was determined on the basis of spectroscopic analysis, and stereostructure of the amino acids were deduced by Marfey's method. Compound 1 showed inhibitory activity against migration of HeLa cells in the ranges of 0.1-10 μM concentration through both wound-healing assay and chemotaxicell chamber assay, while the cell viability was maintained more than 75% up to 10 μM concentration of 1.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Movement / drug effects
  • Cell Survival / drug effects
  • HeLa Cells
  • Humans
  • Magnetic Resonance Spectroscopy
  • Molecular Structure
  • Peptides, Cyclic / chemistry*
  • Peptides, Cyclic / isolation & purification
  • Peptides, Cyclic / pharmacology*
  • Porifera / chemistry*
  • Proline / chemistry*

Substances

  • Peptides, Cyclic
  • stylissamide X
  • Proline