Abstract
Frequent downregulation of microRNA-449a (miR-449a) was detected in 14 human bladder cancer tissues. The restoration of miR-449a inhibited cell growth and induced G1-phase arrest in T24 and 5637 human bladder cancer cells. CDK6 and CDC25a were downregulated after miR-449a treatment, resulting in the functional accumulation of the pocket proteins Rb and p130. The growth of T24 tumor xenografts was suppressed by exogenous miR-449a, and the nuclear proliferation antigen Ki-67 was downregulated in miR-449a-treated tumors. These results suggest a tumor-suppressive role for miR-449a in human bladder cancer.
Copyright © 2012 Elsevier Ireland Ltd. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aged
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Aged, 80 and over
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Animals
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Base Sequence
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Cell Growth Processes / genetics
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Cell Line, Tumor
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Crk-Associated Substrate Protein / genetics*
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Crk-Associated Substrate Protein / metabolism
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Cyclin-Dependent Kinase 6 / antagonists & inhibitors
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Down-Regulation
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Female
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G1 Phase Cell Cycle Checkpoints / genetics
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Gene Expression Regulation, Neoplastic
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Humans
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Male
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Mice
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Mice, Inbred BALB C
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MicroRNAs / administration & dosage
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MicroRNAs / biosynthesis
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MicroRNAs / genetics*
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Middle Aged
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Mitochondrial Proteins
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Molecular Sequence Data
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Phosphorylation
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Retinoblastoma Protein / genetics*
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Retinoblastoma Protein / metabolism
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Transplantation, Heterologous
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Urinary Bladder Neoplasms / genetics*
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Urinary Bladder Neoplasms / metabolism
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Urinary Bladder Neoplasms / pathology
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cdc25 Phosphatases / antagonists & inhibitors
Substances
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BCAR1 protein, human
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Crk-Associated Substrate Protein
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MIRN449 microRNA, human
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MicroRNAs
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Mitochondrial Proteins
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Retinoblastoma Protein
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SPATA18 protein, human
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CDK6 protein, human
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Cyclin-Dependent Kinase 6
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CDC25A protein, human
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cdc25 Phosphatases