A label-free fluorescence immunoassay system for the sensitive detection of the mycotoxin, ochratoxin A

Taihua Li; Eun-Jung Jo; Min-Gon Kim

doi:10.1039/c2cc17088d

A label-free fluorescence immunoassay system for the sensitive detection of the mycotoxin, ochratoxin A

Chem Commun (Camb). 2012 Feb 25;48(17):2304-6. doi: 10.1039/c2cc17088d. Epub 2012 Jan 23.

Authors

Taihua Li¹, Eun-Jung Jo, Min-Gon Kim

Affiliation

¹ Advanced Photonics Research Institute (APRI), Gwangju Institute of Science and Technology (GIST), Gwangju 500-712, South Korea.

PMID: 22266737
DOI: 10.1039/c2cc17088d

Abstract

A label-free fluorescence immunoassay system relying on fluorescence from the dianionic form of ochratoxin A (OTA) in the OTA/anti-OTA complex was developed. With an optimized system, the fluorescence immunoassay can be used to detect OTA in a highly specific manner with a detection limit of 0.5 ng mL(-1).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies / chemistry
Antibodies / immunology
Fluorescence Resonance Energy Transfer
Immunoassay*
Ochratoxins / analysis*

Substances

Antibodies
Ochratoxins
ochratoxin A