Abstract
Humans develop polyclonal, serotype-specific neutralizing antibody responses after dengue virus (DENV) infection. Many mouse antibodies that neutralize DENV bind to the lateral ridge or A strand epitopes on domain III of the viral envelope (EDIII) protein. It has been assumed that these epitopes are also the main target of human neutralizing antibodies. Using recombinant dengue serotype 2 viruses with altered EDIII epitopes, we demonstrate that EDIII epitopes are not the main target of human neutralizing antibody.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Animals
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Antibodies, Neutralizing / immunology*
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Antibodies, Viral / immunology
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Dengue / immunology*
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Dengue / virology
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Dengue Virus / chemistry
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Dengue Virus / genetics
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Dengue Virus / immunology*
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Epitopes / genetics
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Epitopes / immunology*
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Humans
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Immune Sera / immunology*
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Mice
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Protein Structure, Tertiary
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Viral Envelope Proteins / chemistry*
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Viral Envelope Proteins / genetics
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Viral Envelope Proteins / immunology*
Substances
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Antibodies, Neutralizing
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Antibodies, Viral
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Epitopes
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Immune Sera
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Viral Envelope Proteins