Development of microsatellite markers for the neotropical tree species Dipteryx alata (Fabaceae)

Am J Bot. 2012 Feb;99(2):e72-3. doi: 10.3732/ajb.1100377. Epub 2012 Jan 26.

Abstract

Premise of the study: Microsatellite markers were developed for the population genetic analyses of the neotropical tree Dipteryx alata (Fabaceae).

Methods and results: Microsatellites were developed from a genomic shotgun library. Polymorphism at each microsatellite loci was analyzed based on 94 individuals from three populations. Eight loci amplified successfully and presented one to 10 alleles, and expected heterozygosities ranged from 0.097 to 0.862. Four loci also amplified in Pterodon emarginatus and presented similar polymorphism.

Conclusion: The eight microsatellite primer pairs are potentially suitable for population genetic studies and successfully amplified in another Fabaceae species.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • DNA Primers / genetics*
  • DNA, Plant / genetics
  • Dipteryx / genetics*
  • Gene Frequency
  • Genetic Loci
  • Genomic Library
  • Genotyping Techniques
  • Heterozygote
  • Microsatellite Repeats*
  • Polymorphism, Genetic

Substances

  • DNA Primers
  • DNA, Plant