Polymorphisms in the CC-chemokine receptor-2 (CCR2) and -5 (CCR5) genes and risk of myocardial infarction among Tunisian male patients

Clin Biochem. 2012 Apr;45(6):420-4. doi: 10.1016/j.clinbiochem.2012.01.004. Epub 2012 Jan 20.

Abstract

Objectives: The aim of the present study was to investigate the association between CCR2-Val64Ile and CCR5-Δ32 variants and the estimation of haplotypes with MI in a sample of the Tunisian population.

Design and methods: A total of 290 unrelated MI patients and 282 healthy controls were studied. The CCR2-Val64Ile and CCR5-Δ32 variants were analyzed by PCR-RFLP.

Results: Subjects carrying at least one copy of the CCR5-deletion allele were significantly more common in the control group, suggesting an atheroprotective effect (adjusted OR=0.44, 95% CI=0.28-0.72, p=0.001). Haplotype analysis showed that MI patients had significantly less 64Val-Del haplotype (9.9% vs. 21.3%, OR=0.30, 95% CI=0.21-0.43, p<0.001) and 64Ile-Ins haplotype (12.3% vs. 16.7%, OR=0.58, 95% CI=0.42-0.80, p<0.001).

Conclusion: A protective effect of the CCR5-Δ32 polymorphism against MI in the Tunisian population was found.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Case-Control Studies
  • DNA Mutational Analysis
  • Gene Frequency
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Haplotypes
  • Humans
  • Linkage Disequilibrium
  • Male
  • Middle Aged
  • Myocardial Infarction / genetics*
  • Polymorphism, Genetic*
  • Polymorphism, Restriction Fragment Length
  • Receptors, CCR2 / genetics*
  • Receptors, CCR5 / genetics*
  • Risk Factors
  • Tunisia

Substances

  • CCR2 protein, human
  • Receptors, CCR2
  • Receptors, CCR5