Secretory leukocyte protease inhibitor (SLPI) might contaminate murine monoclonal antibodies after purification on protein G

J Biotechnol. 2012 Mar 31;158(1-2):34-5. doi: 10.1016/j.jbiotec.2011.12.025. Epub 2012 Jan 20.

Abstract

The large scale production of a monoclonal anti-progesterone antibody in serum free medium followed by affinity chromatography on protein G lead to a contamination of the antibody sample with a protein of about 14 kDa. This protein was identified by mass spectrometry as secretory leukocyte protease inhibitor (SLPI). This SLPI contamination lead to a failure of the fiber-optic based competitive fluorescence assay to detect progesterone in milk. Purification of the monoclonal antibody using protein A columns circumvented this problem.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Anti-Idiotypic / immunology
  • Antibodies, Anti-Idiotypic / isolation & purification*
  • Antibodies, Monoclonal / immunology
  • Antibodies, Monoclonal / isolation & purification*
  • Bacterial Proteins / chemistry
  • Chromatography, Affinity / methods
  • Mice
  • Milk / chemistry
  • Progesterone / immunology*
  • Secretory Leukocyte Peptidase Inhibitor / chemistry
  • Secretory Leukocyte Peptidase Inhibitor / isolation & purification*
  • Staphylococcal Protein A / chemistry

Substances

  • Antibodies, Anti-Idiotypic
  • Antibodies, Monoclonal
  • Bacterial Proteins
  • IgG Fc-binding protein, Streptococcus
  • Secretory Leukocyte Peptidase Inhibitor
  • Staphylococcal Protein A
  • Progesterone