Plasmid-mediate transfer of FLO1 into industrial Saccharomyces cerevisiae PE-2 strain creates a strain useful for repeat-batch fermentations involving flocculation-sedimentation

Bioresour Technol. 2012 Mar:108:162-8. doi: 10.1016/j.biortech.2011.12.089. Epub 2011 Dec 24.

Abstract

The flocculation gene FLO1 was transferred into the robust industrial strain Saccharomyces cerevisiae PE-2 by the lithium acetate method. The recombinant strain showed a fermentation performance similar to that of the parental strain. In 10 repeat-batch cultivations in VHG medium with 345 g glucose/L and cell recycling by flocculation-sedimentation, an average final ethanol concentration of 142 g/L and an ethanol productivity of 2.86 g/L/h were achieved. Due to the flocculent nature of the recombinant strain it is possible to reduce the ethanol production cost because of lower centrifugation and distillation costs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ethanol / metabolism*
  • Fermentation
  • Flocculation
  • Gene Transfer Techniques*
  • Industrial Microbiology / methods*
  • Mannose-Binding Lectins / genetics*
  • Plasmids / genetics
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism*
  • Saccharomyces cerevisiae Proteins / genetics*
  • Species Specificity
  • Transformation, Genetic / genetics*

Substances

  • FLO1 protein, S cerevisiae
  • Mannose-Binding Lectins
  • Saccharomyces cerevisiae Proteins
  • Ethanol