Abstract
Neuramide, a tissue extract having antiviral action against influenza A virus and immunostimulant action, was analyzed by preparative size-exclusion high-performance liquid chromatography (HPLC) and a low-molecular-weight fraction responsible for the antiviral action was isolated after reversed-phase HPLC. Four fractions having immunostimulant activity were also isolated, as evidenced by their potentiating action in the human lymphocyte proliferation induced by phytohaemagglutinin.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adjuvants, Immunologic / isolation & purification*
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Antimicrobial Cationic Peptides*
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Antiviral Agents / isolation & purification*
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Cell Division / drug effects
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Cells, Cultured
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Chromatography, High Pressure Liquid
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Humans
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In Vitro Techniques
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Influenza A virus / drug effects
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Leukocytes, Mononuclear / drug effects
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Lymphocytes / drug effects
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Methylene Chloride
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Molecular Weight
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Peptides / analysis*
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Peptides / chemistry
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Peptides / pharmacology
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Ultrafiltration
Substances
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Adjuvants, Immunologic
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Antimicrobial Cationic Peptides
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Antiviral Agents
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Peptides
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Methylene Chloride
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neuramide