Association of TMPRSS6 polymorphisms with ferritin, hemoglobin, and type 2 diabetes risk in a Chinese Han population

Am J Clin Nutr. 2012 Mar;95(3):626-32. doi: 10.3945/ajcn.111.025684. Epub 2012 Feb 1.

Abstract

Background: Transmembrane protease serine 6 (TMPRSS6) regulates iron homeostasis by inhibiting the expression of hepcidin. Multiple common variants in TMPRSS6 were significantly associated with serum iron in recent genome-wide association studies, but their effects in the Chinese remain to be elucidated.

Objective: The objective was to determine whether the TMPRSS6 single nucleotide polymorphisms (SNPs) rs855791(V736A) and rs4820268(D521D) were associated with blood hemoglobin and plasma ferritin concentrations and risk of type 2 diabetes in Chinese individuals.

Design: The SNPs rs855791(V736A) and rs4820268(D521D) in the TMPRSS6 gene were genotyped and tested for their associations with plasma iron and type 2 diabetes risk in 1574 unrelated Chinese Hans from Beijing.

Results: The 2 TMPRSS6 SNPs rs855791(V736A) and rs4820268(D521D) were both significantly associated with plasma ferritin (P ≤ 0.0058), hemoglobin (P ≤ 0.0013), iron overload risk (P ≤ 0.0068), and type 2 diabetes risk (P ≤ 0.0314). None of the associations with hemoglobin or plasma ferritin remained significant (P ≥ 0.1229) when the 2 variants were both included in one linear regression model. A haplotype carrying both iron-lowering alleles from the 2 TMPRSS SNPs showed significant associations with lower hemoglobin (P = 0.0014), lower plasma ferritin (P = 0.0027), and a reduced risk of iron overload (P = 0.0017) and of type 2 diabetes (P = 0.0277).

Conclusions: These findings suggest that TMPRSS6 variants were significantly associated with plasma ferritin, hemoglobin, risk of iron overload, and type 2 diabetes in Chinese Hans. The type 2 diabetes risk conferred by the TMPRSS6 SNPs is possibly mediated by plasma ferritin.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Antimicrobial Cationic Peptides / antagonists & inhibitors
  • Antimicrobial Cationic Peptides / genetics
  • Antimicrobial Cationic Peptides / metabolism
  • Asian People / genetics
  • China
  • Cohort Studies
  • Diabetes Mellitus, Type 2 / genetics*
  • Female
  • Ferritins / blood*
  • Genome-Wide Association Study
  • Genotype
  • Hemoglobins / analysis*
  • Hemoglobins / metabolism
  • Hepcidins
  • Homeostasis
  • Humans
  • Iron / blood
  • Linear Models
  • Male
  • Membrane Proteins / genetics*
  • Mendelian Randomization Analysis
  • Middle Aged
  • Polymorphism, Single Nucleotide*
  • Risk Factors
  • Serine Endopeptidases / genetics*

Substances

  • Antimicrobial Cationic Peptides
  • HAMP protein, human
  • Hemoglobins
  • Hepcidins
  • Membrane Proteins
  • Ferritins
  • Iron
  • Serine Endopeptidases
  • TMPRSS6 protein, human