Advenella faeciporci sp. nov., a nitrite-denitrifying bacterium isolated from nitrifying-denitrifying activated sludge collected from a laboratory-scale bioreactor treating piggery wastewater

Int J Syst Evol Microbiol. 2012 Dec;62(Pt 12):2986-2990. doi: 10.1099/ijs.0.037440-0. Epub 2012 Feb 3.

Abstract

Strain M-07(T) was isolated from nitrifying-denitrifying activated sludge treating piggery wastewater. Phylogenetic analysis based on 16S rRNA gene sequences demonstrated that strain M-07(T) belonged to the genus Advenella. 16S rRNA gene sequence similarity between M-07(T) and Advenella incenata CCUG 45225(T), Advenella mimigardefordensis DPN7(T) and Advenella kashmirensis WT001(T) was 96.5, 97.3 and 96.9%, respectively. The DNA G+C content of strain M-07(T) was 49.5 mol%, which was approximately 5 mol% lower than the range for the genus Advenella (53.5-58.0 mol%). The predominant cellular fatty acids of strain M-07(T) were C(16:0), summed feature 3 (comprising C(16:1)ω7c and/or iso-C(15:0) 2-OH), C(17:0) cyclo and summed feature 2 (comprising one or more of C(14:0) 3-OH, iso-C(16:1) I, an unidentified fatty acid with an equivalent chain-length of 10.928 and C(12:0) alde). The isoprenoid quinone was Q-8. On the basis of phenotypic characteristics, phylogenetic analysis and DNA-DNA relatedness, strain M-07(T) should be classified as a novel species of the genus Advenella, for which the name Advenella faeciporci sp. nov. is proposed. The type strain is M-07(T) ( = JCM 17746(T) = KCTC 23732(T)).

MeSH terms

  • Alcaligenaceae / classification*
  • Alcaligenaceae / genetics
  • Alcaligenaceae / isolation & purification
  • Animals
  • Bacterial Typing Techniques
  • Base Composition
  • Bioreactors
  • DNA, Bacterial / genetics
  • Denitrification
  • Fatty Acids / analysis
  • Molecular Sequence Data
  • Nitrification
  • Nitrites
  • Phylogeny*
  • Quinones / analysis
  • RNA, Ribosomal, 16S / genetics
  • Sequence Analysis, DNA
  • Sewage / microbiology*
  • Swine
  • Wastewater / microbiology*

Substances

  • DNA, Bacterial
  • Fatty Acids
  • Nitrites
  • Quinones
  • RNA, Ribosomal, 16S
  • Sewage
  • Waste Water

Associated data

  • GENBANK/AB567741