Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry

Victor V Fadeev; Maxim Y Gorbunov; Timofey S Gostev

doi:10.1002/jbio.201100113

Studying photoprotective processes in the green alga Chlorella pyrenoidosa using nonlinear laser fluorimetry

J Biophotonics. 2012 Jul;5(7):502-7. doi: 10.1002/jbio.201100113. Epub 2012 Feb 6.

Authors

Victor V Fadeev¹, Maxim Y Gorbunov, Timofey S Gostev

Affiliation

¹ Physics Department, M. V. Lomonosov Moscow State University, Leninskie Gory, 1, Block 2, GSP-1, 119991 Moscow, Russia.

PMID: 22308058
DOI: 10.1002/jbio.201100113

Abstract

We use an advanced fluorescence method of Nonlinear Laser Fluorimetry in combination with Fluorescence Induction and Relaxation technique to study the influence of excess-light conditions on the physiological state of the green alga Chlorella pyrenoidosa. We demonstrate that zeaxanthin-dependent non-photochemical quenching leads to a significant increase in the rate constant of singlet-singlet annihilation of chlorophyll a excited state, which suggests profound conformational changes in the light-harvesting complexes of photosystem II.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chlorella / cytology
Chlorella / metabolism*
Chlorella / radiation effects*
Darkness
Fluorometry / methods*
Kinetics
Lasers*
Light / adverse effects*
Stress, Physiological / radiation effects
Xanthophylls / metabolism
Zeaxanthins

Substances

Xanthophylls
Zeaxanthins