Whole-exome sequencing identifies mutations in GPR179 leading to autosomal-recessive complete congenital stationary night blindness

Am J Hum Genet. 2012 Feb 10;90(2):321-30. doi: 10.1016/j.ajhg.2011.12.007.

Abstract

Congenital stationary night blindness (CSNB) is a heterogeneous retinal disorder characterized by visual impairment under low light conditions. This disorder is due to a signal transmission defect from rod photoreceptors to adjacent bipolar cells in the retina. Two forms can be distinguished clinically, complete CSNB (cCSNB) or incomplete CSNB; the two forms are distinguished on the basis of the affected signaling pathway. Mutations in NYX, GRM6, and TRPM1, expressed in the outer plexiform layer (OPL) lead to disruption of the ON-bipolar cell response and have been seen in patients with cCSNB. Whole-exome sequencing in cCSNB patients lacking mutations in the known genes led to the identification of a homozygous missense mutation (c.1807C>T [p.His603Tyr]) in one consanguineous autosomal-recessive cCSNB family and a homozygous frameshift mutation in GPR179 (c.278delC [p.Pro93Glnfs(∗)57]) in a simplex male cCSNB patient. Additional screening with Sanger sequencing of 40 patients identified three other cCSNB patients harboring additional allelic mutations in GPR179. Although, immunhistological studies revealed Gpr179 in the OPL in wild-type mouse retina, Gpr179 did not colocalize with specific ON-bipolar markers. Interestingly, Gpr179 was highly concentrated in horizontal cells and Müller cell endfeet. The involvement of these cells in cCSNB and the specific function of GPR179 remain to be elucidated.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alleles
  • Animals
  • Electroretinography / methods
  • Exome*
  • Eye Diseases, Hereditary
  • Female
  • Genetic Diseases, X-Linked
  • Genetic Heterogeneity
  • Genotyping Techniques / methods
  • Heterozygote
  • Homozygote
  • Humans
  • Male
  • Mice
  • Mutation*
  • Myopia / genetics*
  • Night Blindness / genetics*
  • Phenotype
  • Polymorphism, Single Nucleotide
  • Protein Structure, Tertiary
  • Proteoglycans / genetics
  • Receptors, G-Protein-Coupled / genetics*
  • Receptors, Metabotropic Glutamate / genetics
  • Retina / abnormalities
  • TRPM Cation Channels / genetics

Substances

  • NYX protein, human
  • Proteoglycans
  • Receptors, G-Protein-Coupled
  • Receptors, Metabotropic Glutamate
  • TRPM Cation Channels
  • TRPM1 protein, human
  • metabotropic glutamate receptor 6

Supplementary concepts

  • Night blindness, congenital stationary