A resveratrol analog, phoyunbene B, induces G2/M cell cycle arrest and apoptosis in HepG2 liver cancer cells

Bioorg Med Chem Lett. 2012 Mar 1;22(5):2114-8. doi: 10.1016/j.bmcl.2011.12.095. Epub 2012 Jan 16.

Abstract

Among the seven natural resveratrol analogs separated and identified from Pholidota yunnanensis R(OLFE), we found phoyunbene B (PYB, trans-3,4'-dihydroxy-2',3',5-trimethoxystilbene) was more effective in inhibiting the growth of HepG2 hepatocellular carcinoma cells than resveratrol. The inhibitory effect of PYB in HepG2 cells was due to its induction of G2/M cell cycle arrest and apoptosis. Induction of G2/M phase cell cycle arrest by PYB was associated with its up-regulation of Cyclin B1, while its induction of apoptosis was accompanied with its down-regulation of Bcl-2 and up-regulation of Bax. Our in vitro invasion/migration assays also showed that PYB could inhibit the invasion of hepatocellular carcinoma cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antineoplastic Agents, Phytogenic / chemistry*
  • Antineoplastic Agents, Phytogenic / isolation & purification
  • Antineoplastic Agents, Phytogenic / pharmacology*
  • Apoptosis / drug effects
  • Carcinoma, Hepatocellular / drug therapy*
  • G2 Phase Cell Cycle Checkpoints / drug effects*
  • Hep G2 Cells
  • Humans
  • Liver Neoplasms / drug therapy*
  • M Phase Cell Cycle Checkpoints / drug effects
  • Orchidaceae / chemistry
  • Resveratrol
  • Stilbenes / chemistry*
  • Stilbenes / isolation & purification
  • Stilbenes / pharmacology*

Substances

  • Antineoplastic Agents, Phytogenic
  • Stilbenes
  • trans-3,4'-dihydroxy-2',3',5-trimethoxystilbene
  • Resveratrol