[Induction of repair enzyme O6-methylguanine-DNA methyltransferase gene expression under the influence of cytokine EMAP II in human cells in vitro]

Tsitol Genet. 2011 Nov-Dec;45(6):53-60.
[Article in Russian]

Abstract

The aim of our study was to investigate the effect of recombinant human cytokine EMAP II (endothelial monocyte-activating polypeptide II) on the expression of MGMT gene, encoding repair enzyme O6-methylguanine-DNA methyltransferase (MGMT) in human cell cultures. The influence of EMAP II on cell proliferation was performed using routine MTT assay. Identification of MGMT in cell extracts was performed using Western blot analysis. We used cell lines: A102 (fibroblasts), CB-1 (umbilical cord blood stromal cells), 4BL6 (cells derived from peripheral blood). It was shown that cytokine EMAP II caused induction of MGMT expression in studied human cell lines. There was a decrease in cell number at high concentrations of this cytokine. It was found that the presence of cytokine EMAP II in serum-free growth medium leads to increasing of repair enzyme MGMT expression level in human cells in vitro.

Publication types

  • English Abstract

MeSH terms

  • Antineoplastic Agents, Alkylating / administration & dosage
  • Antineoplastic Agents, Alkylating / adverse effects*
  • Blotting, Western
  • Cell Line, Tumor
  • Cell Proliferation / drug effects
  • Cell Survival / drug effects*
  • Culture Media, Serum-Free
  • Cytokines* / pharmacology
  • Cytokines* / therapeutic use
  • DNA Damage / drug effects
  • DNA Repair / drug effects*
  • Enzyme Induction / drug effects
  • Fetal Blood / cytology
  • Fibroblasts / cytology
  • Fibroblasts / drug effects
  • Fibroblasts / metabolism
  • Gene Expression / drug effects*
  • Guanine / analogs & derivatives
  • Guanine / metabolism
  • Hepatocytes / cytology
  • Hepatocytes / drug effects
  • Hepatocytes / metabolism
  • Humans
  • Methylation
  • Neoplasm Proteins* / pharmacology
  • Neoplasm Proteins* / therapeutic use
  • Neoplasms / drug therapy
  • Neoplasms / pathology
  • O(6)-Methylguanine-DNA Methyltransferase / genetics
  • O(6)-Methylguanine-DNA Methyltransferase / metabolism*
  • RNA-Binding Proteins* / pharmacology
  • RNA-Binding Proteins* / therapeutic use
  • Stromal Cells / cytology
  • Stromal Cells / drug effects
  • Stromal Cells / metabolism

Substances

  • Antineoplastic Agents, Alkylating
  • Culture Media, Serum-Free
  • Cytokines
  • Neoplasm Proteins
  • RNA-Binding Proteins
  • small inducible cytokine subfamily E, member 1
  • Guanine
  • O-(6)-methylguanine
  • O(6)-Methylguanine-DNA Methyltransferase