Comparative transcriptional profiling in HIV-infected patients using human stress arrays: clues to metabolic syndrome

In Vivo. 2012 Mar-Apr;26(2):237-42.

Abstract

Highly active antiretroviral therapy (HAART therapy) for HIV-1 infection has significantly increased the survival and quality of life of patients with this disease. However, in several epidemiological studies the onset of metabolic syndrome is a phenomenon reported to be extremely frequent. In the present study, genes involved in the molecular cascade responsible for the alteration of fat tissue and of lipid and glucose metabolism in patients with HIV-1 infection treated with antiretroviral therapy were identified. Towards this goal, hybridization using Atlas cDNA Expression Arrays allowed simultaneous monitoring of the expression levels of approximately 250 genes and identification of a panel of changes in relation to different therapeutic groups and in the presence of metabolic syndrome, with some genes being up-regulated, while others are down-regulated in the different subgroups of patients. The results of this analysis have shown a panel of transcriptional changes associated with oxidative stress mechanisms that provide a basis for further studies on understanding of mechanisms that, in vivo, are the foundation the metabolic disorders in patients with HIV infection.

Publication types

  • Comparative Study

MeSH terms

  • Adipogenesis / drug effects
  • Adipogenesis / genetics
  • Anti-HIV Agents / adverse effects
  • Anti-HIV Agents / pharmacology*
  • Antiretroviral Therapy, Highly Active / adverse effects*
  • CCAAT-Enhancer-Binding Proteins / metabolism
  • DNA, Complementary / genetics
  • Gene Expression Profiling*
  • HIV Infections / genetics*
  • HIV Protease Inhibitors / adverse effects
  • HIV Protease Inhibitors / pharmacology
  • HIV-1*
  • HIV-Associated Lipodystrophy Syndrome / chemically induced
  • HIV-Associated Lipodystrophy Syndrome / epidemiology
  • HIV-Associated Lipodystrophy Syndrome / metabolism
  • Humans
  • Incidence
  • Metabolic Syndrome / chemically induced*
  • Metabolic Syndrome / epidemiology
  • Metabolic Syndrome / genetics
  • Oxidative Stress / drug effects
  • Oxidative Stress / genetics
  • Peroxisome Proliferator-Activated Receptors / metabolism
  • Transcription, Genetic / drug effects*

Substances

  • Anti-HIV Agents
  • CCAAT-Enhancer-Binding Proteins
  • DNA, Complementary
  • HIV Protease Inhibitors
  • Peroxisome Proliferator-Activated Receptors