Diverse sequence types of Klebsiella pneumoniae contribute to the dissemination of blaNDM-1 in India, Sweden, and the United Kingdom

Antimicrob Agents Chemother. 2012 May;56(5):2735-8. doi: 10.1128/AAC.06142-11. Epub 2012 Feb 21.

Abstract

Clinical isolates of Klebsiella pneumoniae producing NDM-1 carbapenemase from India (n = 22), the United Kingdom (n = 13), and Sweden (n = 4) were subjected to multilocus sequence typing (MLST), automated repetitive sequence-based PCR (rep-PCR), serotyping, virulence gene screening, and plasmid replicon typing. The most frequently detected MLST sequence types (STs) were ST14 (n = 13; all serotype K2), ST11, ST149, ST231, and ST147. The correlation between MLST and automated rep-PCR was excellent. IncA/C was the most frequently detected plasmid replicon type (n = 14). ST14, ST11, and other successful clones may be important for the dissemination of bla(NDM-1).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Electrophoresis, Gel, Pulsed-Field
  • Gene Transfer, Horizontal
  • Humans
  • India
  • Klebsiella pneumoniae / genetics*
  • Klebsiella pneumoniae / isolation & purification
  • Klebsiella pneumoniae / pathogenicity
  • Multilocus Sequence Typing
  • Phylogeography
  • Plasmids / genetics
  • Polymerase Chain Reaction
  • Replicon / genetics
  • Serotyping
  • Sweden
  • United Kingdom
  • beta-Lactam Resistance / genetics*
  • beta-Lactamases / genetics*

Substances

  • beta-Lactamases
  • beta-lactamase NDM-1