Production of erythropoietin by BHK cells growing on the microcarriers trapped in alginate gel beads

Y Shirai; K Hashimoto; H Kawahara; R Sasaki; K Hitomi; H Chiba

doi:10.1007/BF00386147

Production of erythropoietin by BHK cells growing on the microcarriers trapped in alginate gel beads

Cytotechnology. 1989 May;2(2):141-5. doi: 10.1007/BF00386147.

Authors

Y Shirai¹, K Hashimoto, H Kawahara, R Sasaki, K Hitomi, H Chiba

Affiliation

¹ Department of Chemical Engineering, Faculty of Engineering, Kyoto University, Sakyo-ku, 606, Kyoto, Japan.

PMID: 22358627
DOI: 10.1007/BF00386147

Abstract

Baby hamster kidney (BHK) cells engineered to produce recombinant human erythropoietin (EPO) were cultured at high density on microcarriers entrapped by calcium alginate gel particles. In this system, the BHK cells proliferated not only on the microcarriers but also in vacant spaces in the alginate gel particles. These spaces contributed greatly to high-density cultivation of the cells and a high productivity of EPO.