A more accurate profile of Achyrocline satureioides hypocholesterolemic activity

Cell Biochem Funct. 2012 Jun;30(4):347-53. doi: 10.1002/cbf.2812. Epub 2012 Feb 22.

Abstract

The aim of this study was to investigate the effect of the aqueous extract (AE) of Achyrocline satureioides on serum lipid profile, liver oxidative profile and Na(+),K(+)-ATPase activity of rats submitted to a hyperlipidic diet. The animals were divided into four groups: control (C), AE 10% (A(10)), hyperlipidic (H) and hyperlipidic/AE 10% (HA(10)). In serum, we measured the levels of total cholesterol (TC), high-density lipoprotein, very-low-density lipoprotein, low-density lipoprotein (LDL) and triglyceride (TG). In liver homogenates, we measured the thiobarbituric acid reactive substances, the carbonyl proteins, the non-protein thiols (NPSHs) and the activity of superoxide dismutase, catalase (CAT) and Na(+),K(+)-ATPase. We observed a significant increase in the TC and LDL levels in the H group. A. satureioides prevented these effects, decreased the TG levels in the HA(10) group and increased the NPSH levels in the A(10) and HA(10) groups. The H group showed an increase in the carbonyl protein level and a decrease in CAT and Na(+),K(+)-ATPase activities. With the use of this model, results show that increased levels of lipids are related to a redox imbalance in the liver, which is also related to the inhibition of Na(+),K(+)-ATPase activity, and that chronic administration of the AE of A. satureioides is capable of changing this profile.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Achyrocline / chemistry*
  • Animals
  • Anticholesteremic Agents / pharmacology*
  • Catalase / metabolism
  • Cholesterol / blood
  • Diet, High-Fat
  • Enzyme Activation / drug effects
  • Lipids / blood
  • Lipoproteins, HDL / blood
  • Lipoproteins, LDL / blood
  • Liver / drug effects
  • Liver / enzymology
  • Liver / metabolism
  • Male
  • Plant Extracts / pharmacology*
  • Rats
  • Rats, Wistar
  • Sodium-Potassium-Exchanging ATPase / antagonists & inhibitors
  • Sodium-Potassium-Exchanging ATPase / metabolism
  • Superoxide Dismutase / metabolism
  • Thiobarbituric Acid Reactive Substances / analysis
  • Triglycerides / blood

Substances

  • Anticholesteremic Agents
  • Lipids
  • Lipoproteins, HDL
  • Lipoproteins, LDL
  • Plant Extracts
  • Thiobarbituric Acid Reactive Substances
  • Triglycerides
  • Cholesterol
  • Catalase
  • Superoxide Dismutase
  • Sodium-Potassium-Exchanging ATPase