Analysis of AGE modified proteins and RAGE expression in HER2/neu negative invasive ductal carcinoma

Biochem Biophys Res Commun. 2012 Mar 16;419(3):490-4. doi: 10.1016/j.bbrc.2012.02.039. Epub 2012 Feb 14.

Abstract

Cancer is associated with increased glycolysis and carbonyl stress. In view of this, AGE modified proteins were identified from clinical breast cancer tissue using 2DE-immunoblot and mass-spectrometry. These proteins were identified to be serotransferrin, fibrinogen gamma chain, glycerol-3-phosphate dehydrogenase, lactate dehydrogenase, annexin II, prohibitin and peroxiredoxin 6, which have established role in cancer. Further, RAGE expression and its downstream signaling proteins NADPH oxidase and NF-kB were studied. Role of these AGE modified proteins and RAGE signaling in breast cancer is discussed.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Breast Neoplasms / metabolism
  • Breast Neoplasms / pathology*
  • Carcinoma, Ductal, Breast / metabolism
  • Carcinoma, Ductal, Breast / pathology*
  • Female
  • Glycation End Products, Advanced / metabolism*
  • Humans
  • Mitogen-Activated Protein Kinases / biosynthesis*
  • Molecular Sequence Data
  • NADPH Oxidases / metabolism
  • NF-kappa B / metabolism
  • Neoplasm Invasiveness
  • Neoplasm Proteins / metabolism*
  • Protein Processing, Post-Translational
  • Receptor for Advanced Glycation End Products / biosynthesis*
  • Receptor, ErbB-2 / analysis
  • Receptor, ErbB-2 / metabolism

Substances

  • Glycation End Products, Advanced
  • NF-kappa B
  • Neoplasm Proteins
  • Receptor for Advanced Glycation End Products
  • NADPH Oxidases
  • Receptor, ErbB-2
  • Mitogen-Activated Protein Kinases