A method for the specific detection of resident bacteria in brine shrimp larvae

Yufeng Niu; Tom Defoirdt; Anamaria Rekecki; Peter De Schryver; Wim Van den Broeck; Shuanglin Dong; Patrick Sorgeloos; Nico Boon; Peter Bossier

doi:10.1016/j.mimet.2012.02.004

A method for the specific detection of resident bacteria in brine shrimp larvae

J Microbiol Methods. 2012 Apr;89(1):33-7. doi: 10.1016/j.mimet.2012.02.004. Epub 2012 Feb 16.

Authors

Yufeng Niu¹, Tom Defoirdt, Anamaria Rekecki, Peter De Schryver, Wim Van den Broeck, Shuanglin Dong, Patrick Sorgeloos, Nico Boon, Peter Bossier

Affiliation

¹ Laboratory of Aquaculture & Artemia Reference Center, Ghent University, Rozier 44, 9000 Ghent, Belgium.

PMID: 22366463
DOI: 10.1016/j.mimet.2012.02.004

Abstract

In this study, we describe an easy but efficient method to specifically target the intestinal resident microbiota in brine shrimp larvae during DGGE analysis, hereby excluding the interference of both transient (luminal) bacteria and body surface bacteria. This effective technique has several advantages over alternative methods, with respect of ease of use and rapidity.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Artemia / microbiology*
Bacteria / genetics
Bacteria / isolation & purification*
DNA Fingerprinting / methods*
Denaturing Gradient Gel Electrophoresis / methods*
Gastrointestinal Tract / microbiology
Larva / microbiology
Time Factors