Involvement of dopaminergic and serotonergic systems in the neurobehavioral toxicity of lambda-cyhalothrin in developing rats

Toxicol Lett. 2012 May 20;211(1):1-9. doi: 10.1016/j.toxlet.2012.02.012. Epub 2012 Feb 24.

Abstract

In view of extensive uses of lambda-cyhalothrin, a new generation type II synthetic pyrethroid, human exposure is quite imminent. The present study has therefore been carried out to investigate effect of lambda-cyhalothrin on brain dopaminergic and serotonergic systems and functional alterations associated with them. Post-lactational exposure to lambda-cyhalothrin (1.0 mg/kg or 3.0 mg/kg body weight, p.o.) from PD22 to PD49 caused a significant decrease in the motor activity and rota-rod performance in rats on PD50 as compared to controls. Decrease in motor activity in lambda-cyhalothrin treated rats was found to persist 15 days after withdrawal of exposure on PD65 while a trend of recovery in rota-rod performance was observed. A decrease in the binding of ³H-Spiperone, known to label dopamine-D2 receptors in corpus striatum associated with decreased expression of tyrosine hydroxylase (TH)-immunoreactivity and TH protein was observed in lambda-cyhalothrin treated rats on PD50 and PD65 compared to controls. Increase in the binding of ³H-Ketanserin, known to label serotonin-2A receptors in frontal cortex was observed in lambda-cyhalothrin exposed rats on PD50 and PD65 as compared to respective controls. The changes were more marked in rats exposed to lambda-cyhalothrin at a higher dose (3.0 mg/kg) and persisted even 15 days after withdrawal of exposure. The results exhibit vulnerability of developing rats to lambda-cyhalothrin and suggest that striatal dopaminergic system is a target of lambda-cyhalothrin. Involvement of serotonin-2A receptors in the neurotoxicity of lambda-cyhalothrin is also suggested. The results further indicate that neurobehavioral changes may be more intense in case exposure to lambda-cyhalothrin continues.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western
  • Brain / drug effects
  • Corpus Callosum / chemistry
  • Corpus Callosum / drug effects
  • Dopaminergic Neurons / drug effects*
  • Dose-Response Relationship, Drug
  • Female
  • Frontal Lobe / chemistry
  • Frontal Lobe / drug effects
  • Motor Activity / drug effects*
  • Nitriles / toxicity*
  • Psychomotor Performance / drug effects
  • Pyrethrins / toxicity*
  • Rats
  • Rats, Wistar
  • Receptor, Serotonin, 5-HT2A / analysis
  • Receptors, Dopamine D2 / analysis
  • Serotonergic Neurons / drug effects*

Substances

  • Nitriles
  • Pyrethrins
  • Receptor, Serotonin, 5-HT2A
  • Receptors, Dopamine D2
  • cyhalothrin