Abstract
We sequenced the full lengths of the gyrA, gyrB, parC, and parE genes in 13 fluoroquinolone-resistant Ureaplasma isolates (levofloxacin MICs, 4 to 32 μg/ml) and 10 susceptible isolates (MICs ≤ 2 μg/ml). Mutations were detected in all resistant isolates but in none of the susceptible isolates. The most prevalent mutation was the S83L substitution in the ParC protein. No plasmid-mediated fluoroquinolone resistance genes were detected.
Publication types
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Research Support, N.I.H., Extramural
MeSH terms
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Anti-Bacterial Agents / pharmacology*
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Chromosomes, Bacterial / genetics*
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DNA Gyrase / genetics
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DNA Topoisomerase IV / genetics
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Drug Resistance, Bacterial / genetics
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Humans
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Isoenzymes / genetics
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Levofloxacin*
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Longitudinal Studies
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Microbial Sensitivity Tests
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Mutation
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Ofloxacin / pharmacology*
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Sequence Analysis, DNA
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United States
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Ureaplasma / drug effects
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Ureaplasma / genetics*
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Ureaplasma / isolation & purification
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Ureaplasma Infections / microbiology
Substances
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Anti-Bacterial Agents
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Isoenzymes
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Levofloxacin
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Ofloxacin
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DNA Topoisomerase IV
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DNA Gyrase