ERCC1 gene +262A/C polymorphism associated with risk of gastric cardiac adenocarcinoma in nonsmokers

Arch Med Res. 2012 Jan;43(1):67-74. doi: 10.1016/j.arcmed.2012.01.010. Epub 2012 Feb 26.

Abstract

Background and aims: Polymorphisms in DNA repair gene may alter an individual's DNA repair capacity and be associated with the risk of various cancers. This study was designed to investigate whether ERCC1 +262A/C and XPF -357A/C polymorphisms affect individual susceptibility to esophageal squamous cell carcinoma (ESCC) and gastric cardiac adenocarcinoma (GCA).

Methods: In 389 ESCC patients vs. 778 healthy controls and 262 GCA patients vs. 524 healthy controls in a high incidence region of northern China, ERCC1 +262A/C polymorphism and XPF -357A/C polymorphism were genotyped by the method of polymerase chain reaction ligase detection reaction (PCR-LDR) and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP) analysis, respectively.

Results: Family history of upper gastrointestinal cancers (UGIC) may increase the risk of ESCC and GCA. Allelotype and genotype distributions of ERCC1 +262A/C and XPF -357A/C polymorphisms in ESCC and GCA patients were not significantly different from that in their respective controls (p >0.05). Compared with ERCC1 +262C/C genotype, A/A genotype decreased the risk of GCA in nonsmokers (age, gender and family history of UGIC adjusted odds ratio [OR] = 0.30, 95% confidence interval [CI] = 0.13-0.70). Neither the A/C nor the C/C genotype was associated with the overall risk of ESCC and GCA when compared with the XPF -357A/A genotype.

Conclusions: ERCC1 +262A/A genotype may reduce the risk of GCA for nonsmokers. XPF -357A/C polymorphism was not associated with the risk of ESCC and GCA in a population of a high-incidence region in northern China.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adenocarcinoma / enzymology
  • Adenocarcinoma / epidemiology
  • Adenocarcinoma / genetics*
  • Aged
  • Base Sequence
  • Carcinoma, Squamous Cell / enzymology
  • Carcinoma, Squamous Cell / epidemiology
  • Carcinoma, Squamous Cell / genetics
  • Cardia / pathology*
  • Case-Control Studies
  • China / epidemiology
  • DNA Mutational Analysis
  • DNA-Binding Proteins / genetics*
  • Endonucleases / genetics*
  • Esophageal Neoplasms / enzymology
  • Esophageal Neoplasms / epidemiology
  • Esophageal Neoplasms / genetics
  • Female
  • Genetic Association Studies
  • Genetic Predisposition to Disease
  • Humans
  • Incidence
  • Male
  • Middle Aged
  • Molecular Sequence Data
  • Polymorphism, Single Nucleotide*
  • Risk Factors
  • Smoking
  • Stomach Neoplasms / enzymology
  • Stomach Neoplasms / epidemiology
  • Stomach Neoplasms / genetics*

Substances

  • DNA-Binding Proteins
  • xeroderma pigmentosum group F protein
  • ERCC1 protein, human
  • Endonucleases