Extraction of high-quality genomic DNA from Ectocarpus

Cold Spring Harb Protoc. 2012 Mar 1;2012(3):365-8. doi: 10.1101/pdb.prot067967.

Abstract

For some applications, such as genome sequencing and high-throughput genotyping with multiple markers, it is necessary to use high-quality genomic DNA. This article describes how to obtain several micrograms of high-quality, cesium chloride-purified DNA from 1 g of Ectocarpus filaments. We also recommend using DNA of this quality for quantitative RT-PCR control reactions. However, simpler, more rapid, kit-based methods are preferable for experiments that involve the treatment of large numbers of individuals, such as genotyping large populations with a small number of markers or PCR screening of large populations.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA / isolation & purification*
  • Molecular Biology / methods*
  • Molecular Typing
  • Phaeophyceae / genetics*
  • Sequence Analysis, DNA

Substances

  • DNA