Erythrocyte antibody screening in solid-phase: a comparison of two solid-phase microplate assays with the indirect antiglobulin test in polyethylene glycol for the detection of irregular erythrocyte antibodies

Vox Sang. 1990;59(2):96-100. doi: 10.1111/j.1423-0410.1990.tb05018.x.

Abstract

The screening of a serum for irregular erythrocyte antibodies in the indirect antiglobulin test is a well-established technique. We compared the test results of two different solid-phase microplate indirect antiglobulin tests with a liquid-phase indirect antiglobulin test in tubes. Antibody screening with both solid-phase microplate techniques proved to be more sensitive than the liquid-phase indirect antiglobulin test. In addition, a difference in sensitivity between the two solid-phase techniques was observed: prior immobilization of test erythrocytes on the microplate followed by incubation with a serum and detection of sensitization with antihuman IgG-coated detector cells gave better test results than secondary immobilization on the microplate of test erythrocytes sensitized with antibodies and an antihuman globulin serum.

Publication types

  • Comparative Study

MeSH terms

  • Antibodies / analysis*
  • Antibody Specificity / immunology
  • Autoantibodies / analysis
  • Coombs Test / instrumentation
  • Erythrocytes / immunology*
  • Hemagglutination / immunology
  • Humans
  • Isoantibodies / analysis
  • Methods
  • Polyethylene Glycols

Substances

  • Antibodies
  • Autoantibodies
  • Isoantibodies
  • Polyethylene Glycols