Mitochondrial proteome analysis reveals depression of the Ndufs3 subunit and activity of complex I in diabetic rat brain

Federica Taurino; Eleonora Stanca; Luisa Siculella; Raffaella Trentadue; Sergio Papa; Franco Zanotti; Antonio Gnoni

doi:10.1016/j.jprot.2012.02.002

Mitochondrial proteome analysis reveals depression of the Ndufs3 subunit and activity of complex I in diabetic rat brain

J Proteomics. 2012 Apr 18;75(8):2331-41. doi: 10.1016/j.jprot.2012.02.002. Epub 2012 Feb 23.

Authors

Federica Taurino¹, Eleonora Stanca, Luisa Siculella, Raffaella Trentadue, Sergio Papa, Franco Zanotti, Antonio Gnoni

Affiliation

¹ Department of Medical Basic Sciences, University of Bari Aldo Moro, Bari, Italy.

PMID: 22387129
DOI: 10.1016/j.jprot.2012.02.002

Abstract

Type-1 diabetes resulting from defective insulin secretion and consequent hyperglycemia, is associated with "diabetic encephalopathy." This is characterized by brain neurophysiological and structural changes resulting in impairment of cognitive function. The present proteomic analysis of brain mitochondrial proteins from streptozotocin-induced type-1 diabetic rats, shows a large decrement of the Ndufs3 protein subunit of complex I, decreased level of the mRNA and impaired catalytic activity of the complex in the diabetic rats as compared to controls. The severe depression of the expression and enzymatic activity of complex I can represent a critical contributing factor to the onset of the diabetic encephalopathy in type-1 diabetes.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Brain / metabolism*
Brain / pathology
Brain Chemistry / physiology
Brain Diseases, Metabolic / etiology
Brain Diseases, Metabolic / metabolism
Brain Diseases, Metabolic / pathology
Diabetes Mellitus, Experimental / chemically induced
Diabetes Mellitus, Experimental / complications
Diabetes Mellitus, Experimental / metabolism*
Diabetes Mellitus, Experimental / pathology
Diabetes Mellitus, Type 1 / chemically induced
Diabetes Mellitus, Type 1 / complications
Diabetes Mellitus, Type 1 / metabolism
Diabetes Mellitus, Type 1 / pathology
Down-Regulation
Electron Transport Complex I / analysis
Electron Transport Complex I / metabolism*
Enzyme Activation
Male
Mitochondrial Proteins / analysis*
Mitochondrial Proteins / metabolism
Models, Biological
NADH Dehydrogenase / analysis
NADH Dehydrogenase / metabolism*
Protein Subunits / analysis
Protein Subunits / metabolism
Proteome / analysis
Proteome / metabolism
Rats
Rats, Wistar
Streptozocin

Substances

Mitochondrial Proteins
Protein Subunits
Proteome
Streptozocin
NADH Dehydrogenase
Electron Transport Complex I
NDUFS3 protein, human