The miR-17/92 cluster is targeted by STAT5 but dispensable for mammary development

Genesis. 2012 Sep;50(9):665-71. doi: 10.1002/dvg.22023. Epub 2012 Mar 31.

Abstract

Genome wide analysis revealed the miR-17/92 cluster as a STAT5 target. This cluster encodes six microRNAs, which predictably target genes that play a role in mammary gland development. In this study, we have deleted the miR-17/92 cluster in mammary stem cells and evaluated in the mouse its function during mammary gland development. Loss of the miR-17/92 cluster did not affect mammary development from prepuberty to lactation. Our studies demonstrated that, while expression of the miR-17/92 cluster is under control of the key mammary transcription factor STAT5, its presence is not required for normal mammary development or lactation.

Publication types

  • Research Support, N.I.H., Intramural

MeSH terms

  • Animals
  • Cells, Cultured
  • Chromatin Immunoprecipitation
  • Epithelial Cells / metabolism
  • Female
  • Gene Deletion
  • Gene Expression Regulation, Developmental / genetics*
  • High-Throughput Nucleotide Sequencing
  • Lactation / genetics
  • Mammary Glands, Animal / cytology
  • Mammary Glands, Animal / growth & development*
  • Mice
  • Mice, Nude
  • MicroRNAs / genetics*
  • Multigene Family / physiology*
  • Pregnancy
  • RNA, Messenger / genetics
  • STAT5 Transcription Factor / genetics*
  • STAT5 Transcription Factor / metabolism
  • Sequence Analysis, DNA
  • Stem Cells

Substances

  • MIRN17-92 microRNA, mouse
  • MicroRNAs
  • RNA, Messenger
  • STAT5 Transcription Factor