Abstract
This study reports a multifunctional electrode approach which directly implements electrokinetic enhancement on a self-assembled-monolayer-based electrochemical sensor for point-of-care diagnostics. Using urinary tract infections as a model system, we demonstrate that electrokinetic enhancement, which involves in situ stirring and heating, can enhance the sensitivity of the strain specific 16S rRNA hybridization assay for 1 order of magnitude and accelerate the time-limiting incubation step with a 6-fold reduction in the incubation time. Since the same electrode platform is used for both electrochemical signal enhancement and electrochemical sensing, the multifunctional electrode approach provides a highly effective strategy toward fully integrated lab-on-a-chip systems for various biomedical applications.
Publication types
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Evaluation Study
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Research Support, N.I.H., Extramural
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Bacteria / genetics
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Bacteria / isolation & purification*
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Biosensing Techniques / instrumentation
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Electrochemical Techniques / instrumentation*
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Electrodes
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Equipment Design
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Escherichia coli / genetics
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Escherichia coli / isolation & purification
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Humans
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Nucleic Acid Hybridization*
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Pseudomonas aeruginosa / genetics
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Pseudomonas aeruginosa / isolation & purification
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RNA, Bacterial / genetics
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RNA, Bacterial / urine*
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RNA, Ribosomal, 16S / genetics
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RNA, Ribosomal, 16S / urine*
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Sensitivity and Specificity
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Staphylococcus saprophyticus / genetics
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Staphylococcus saprophyticus / isolation & purification
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Urinary Tract Infections / diagnosis
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Urinary Tract Infections / urine*
Substances
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RNA, Bacterial
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RNA, Ribosomal, 16S