Preparation of [In-111]-labeled-DTPA-bombesin conjugates at high specific activity and stability: evaluation of labeling parameters and potential stabilizers

P B Pujatti; A V F Massicano; J Mengatti; E B de Araújo

doi:10.1016/j.apradiso.2012.02.064

Preparation of [In-111]-labeled-DTPA-bombesin conjugates at high specific activity and stability: evaluation of labeling parameters and potential stabilizers

Appl Radiat Isot. 2012 May;70(5):856-63. doi: 10.1016/j.apradiso.2012.02.064. Epub 2012 Feb 25.

Authors

P B Pujatti¹, A V F Massicano, J Mengatti, E B de Araújo

Affiliation

¹ Directory of Radiopharmacy, Nuclear and Energy Research Institute, Cidade Universitária da USP - Butantã, São Paulo, Brazil. [email protected]

PMID: 22405643
DOI: 10.1016/j.apradiso.2012.02.064

Abstract

The aim of the present work was to obtain stabilized high specific activity (HSA) (111)In-labeled bombesin conjugates for preclinical evaluations. Parameters influencing the kinetics of labeling were investigated and the effect of stabilizers on HSA radiopeptides stability at room temperature were systematically categorized applying chromatography techniques. A SA of 174 GBq/μmol was achieved with high radiochemical purity, but the labeled compounds exhibited low stability. The addition of stabilizers avoided their radiolysis and significantly increased their stability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bombesin / chemistry*
Chromatography, High Pressure Liquid
Chromatography, Thin Layer
Indium Radioisotopes / chemistry*
Pentetic Acid / chemistry*

Substances

Indium Radioisotopes
Pentetic Acid
Bombesin