Abstract
Cellobiose dehydrogenase (CDH) gene transcripts were quantified by reverse transcription-PCR (RT-PCR) in cultures of Phanerochaete chrysosporium supplemented with various cello- and xylooligosaccharides in order to elucidate the mechanism of enhanced CDH production in xylan/cellulose culture. Cellotriose and cellotetraose induced cdh expression, while xylobiose and xylotriose induced expression of cellobiohydrolase genes, especially cel7C.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carbohydrate Dehydrogenases / biosynthesis*
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Culture Media / chemistry
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Gene Expression Profiling
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Gene Expression Regulation, Fungal*
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Oligosaccharides / metabolism*
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Phanerochaete / enzymology*
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Phanerochaete / genetics
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Phanerochaete / metabolism
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Reverse Transcriptase Polymerase Chain Reaction
Substances
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Culture Media
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Oligosaccharides
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Carbohydrate Dehydrogenases
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cellobiose-quinone oxidoreductase