Surface-engineered polyelectrolyte multilayer capsules: synthetic vaccines mimicking microbial structure and function

Angew Chem Int Ed Engl. 2012 Apr 16;51(16):3862-6. doi: 10.1002/anie.201200048. Epub 2012 Mar 12.

Abstract

Immunizing: to evoke highly potent immune responses against recombinant antigens, hollow capsules consisting of layers of dextran sulphate and poly-L-arginine that encapsulate the antigen ovalbumin (orange circles) were coated with immune-activating CpG-containing oligonucleotides (green). These capsules were readily internalized by dendritic cells and showed activity in further immunization experiments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Capsules / chemistry*
  • Dendritic Cells / immunology
  • Dextran Sulfate / chemistry
  • Electrolytes / chemistry*
  • Interferon-gamma / metabolism
  • Mice
  • Oligodeoxyribonucleotides / chemistry
  • Ovalbumin / genetics
  • Ovalbumin / immunology
  • Ovalbumin / metabolism
  • Peptides / chemistry
  • Recombinant Proteins / genetics
  • Recombinant Proteins / immunology
  • Recombinant Proteins / metabolism
  • Surface Properties
  • Vaccines, Synthetic / chemistry
  • Vaccines, Synthetic / immunology*

Substances

  • CPG-oligonucleotide
  • Capsules
  • Electrolytes
  • Oligodeoxyribonucleotides
  • Peptides
  • Recombinant Proteins
  • Vaccines, Synthetic
  • polyarginine
  • Interferon-gamma
  • Ovalbumin
  • Dextran Sulfate