Persistence of resinous cement residues in dentin treated with different chemical removal protocols

Microsc Res Tech. 2012 Jul;75(7):982-5. doi: 10.1002/jemt.22023. Epub 2012 Mar 14.

Abstract

The aim of this study was to evaluate the persistence of resin cement residues after dentin surface cleaning with different alcohol-based solutions or an essential oil (eucalyptol). Forty bovine teeth were sectioned in order to expose pulp chamber dentin to be washed with 1.0 mL of 2.5% sodium hypochlorite (NaOCl), followed by 0.1 mL of 17% EDTA application for 3 min, and final irrigation with 2.5% NaOCl. The specimens were air dried and resin-based cement was rubbed onto the dentine surface with a microbrush applicator. After 15 min, the surface was scrubbed with a cotton pellet and moistened with different dentin cleaning solutions, compounding the following groups: G1--95% ethanol, G2--70% ethanol, G3--70% isopropyl alcohol, or G4--eucalyptol. The dentin was scrubbed until the cement residues could not be visually detected. Sections were then processed for SEM and evaluated at × 500 magnification. Scores were attributed to each image according to the area covered by residual sealer, and data were subjected to Kruskal-Wallis at 5% significance. Eucalyptol promoted the most adequate dentin cleaning, although no statistical difference was detected amongst the groups (P > 0.05), except between the eucalyptol and 70% ethanol groups (P < 0.05). All the evaluated dentin cleaning solutions were unable to completely remove the cement residues from the dentin surface.

MeSH terms

  • Alcohols / therapeutic use*
  • Animals
  • Cattle
  • Cyclohexanols / therapeutic use*
  • Dental Cements / analysis*
  • Dental Cements / therapeutic use*
  • Dentin / chemistry*
  • Dentin / drug effects*
  • Dentin / ultrastructure
  • Eucalyptol
  • Incisor
  • Microscopy, Electron, Scanning
  • Monoterpenes / therapeutic use*
  • Tooth, Nonvital*

Substances

  • Alcohols
  • Cyclohexanols
  • Dental Cements
  • Monoterpenes
  • Eucalyptol