Abstract
We constructed a mesh-grid integrated microwell array which enables easy trapping and consistent addition of droplets. The grid acts as a microchannel structure to guide droplets into the microwells underneath, and also provides open access for additional manipulation in a high-throughput manner. Each droplet in the array forms a stable environment of pico-litre volume to implement a single-cell-based assay.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Culture Techniques / instrumentation
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Cell Separation / instrumentation
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Escherichia coli / chemistry
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Escherichia coli / isolation & purification
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Green Fluorescent Proteins / chemistry
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High-Throughput Screening Assays / instrumentation
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Microfluidic Analytical Techniques / instrumentation*
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Microfluidic Analytical Techniques / methods
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Single-Cell Analysis / instrumentation*
Substances
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Green Fluorescent Proteins