Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification

Etienne Carbonnelle; Patrick Grohs; Hervé Jacquier; Nesrine Day; Sylvie Tenza; Alexandra Dewailly; Odile Vissouarn; Martin Rottman; Jean-Louis Herrmann; Isabelle Podglajen; Laurent Raskine

doi:10.1016/j.mimet.2012.03.003

Robustness of two MALDI-TOF mass spectrometry systems for bacterial identification

J Microbiol Methods. 2012 May;89(2):133-6. doi: 10.1016/j.mimet.2012.03.003. Epub 2012 Mar 8.

Authors

Etienne Carbonnelle¹, Patrick Grohs, Hervé Jacquier, Nesrine Day, Sylvie Tenza, Alexandra Dewailly, Odile Vissouarn, Martin Rottman, Jean-Louis Herrmann, Isabelle Podglajen, Laurent Raskine

Affiliation

¹ Hôpital Européen Georges Pompidou, Assistance Publique-Hôpitaux de Paris, France. [email protected]

PMID: 22425492
DOI: 10.1016/j.mimet.2012.03.003

Abstract

MALDI-TOF-MS systems (Microflex-Bruker Daltonics/BioTyper™ and Axima-Assurance-Shimadzu/SARAMIS-AnagnosTec) were assessed for bacterial identification. Focusing on bacteria difficult to identify routinely, 296 strains were identified by molecular biology techniques as gold standard. MALDI-TOF-MS identification provided correct results at genus and species level for 94.9%, 83.4% and 83.8%, 65.9% with Biotyper and Saramis respectively.

Publication types

Comparative Study
Evaluation Study

MeSH terms

Bacteria / chemistry*
Bacteria / classification*
Bacteriological Techniques / methods*
Sensitivity and Specificity
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*