Plasma concentrations but not serum concentrations of brain-derived neurotrophic factor are related to pro-inflammatory cytokines in patients undergoing major abdominal surgery

Clin Biochem. 2012 Jun;45(9):631-6. doi: 10.1016/j.clinbiochem.2012.02.025. Epub 2012 Mar 9.

Abstract

Objectives: To investigate peripheral brain-derived neurotrophic factor (BDNF) concentrations in the perioperative period, their relationship with transforming growth factor-β1 (TGF-β1 tumour necrosis factor-α (TNF-α), interleukin-6 (IL-6) and IL-6 genetics.

Design and methods: Prospective, observational study. BDNF, TGF-β1, IL-6 and TNF-α were analysed at baseline (T0), 5 h (T1), 24 h (T2) and 5 days (T3) after surgery, in 21 patients. The IL-6 -174 G/C polymorphism was genotyped.

Results: Serum BDNF concentrations decreased (P=0.048), correlated with TGF-β1 (r=0.610 at T1, r=0.493 at T2, r=0.554 at T3). Plasma BDNF concentrations raised (P=0.049), correlated with IL-6 and TNF-α at T1 (r=0.495 and r=0.441, respectively). BDNF response was predictable from TNF-α and IL-6 concentrations and the IL-6 -174 G/C genotype.

Conclusion: Serum and plasma BDNF concentrations could relate to platelet activation and inflammatory response, respectively. IL-6 genetics played a role in the BDNF acute response.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Abdomen
  • Adult
  • Aged
  • Aged, 80 and over
  • Blood Platelets / immunology
  • Brain-Derived Neurotrophic Factor / blood*
  • Cytokines / blood*
  • Cytokines / immunology
  • Digestive System Surgical Procedures*
  • Female
  • Humans
  • Inflammation / blood*
  • Inflammation / immunology
  • Interleukin-6 / blood
  • Interleukin-6 / genetics
  • Interleukin-6 / immunology
  • Male
  • Middle Aged
  • Perioperative Period
  • Plasma
  • Platelet Activation
  • Polymorphism, Single Nucleotide
  • Serum
  • Transforming Growth Factor beta1 / blood
  • Transforming Growth Factor beta1 / immunology
  • Tumor Necrosis Factor-alpha / blood
  • Tumor Necrosis Factor-alpha / immunology

Substances

  • Brain-Derived Neurotrophic Factor
  • Cytokines
  • Interleukin-6
  • Transforming Growth Factor beta1
  • Tumor Necrosis Factor-alpha