Abstract
A protocol is presented for the high-throughput (HT) production of lyotropic liquid crystalline phases from libraries of lipids and lipid mixtures using standard liquid dispensing robotics, implementing methods that circumvent the problems traditionally associated with handling the highly viscous cubic phase. In addition, the ability to structurally characterize lipidic phases and assess functionality for membrane proteins contained within cubic phases, in a HT manner, is demonstrated. The techniques are combined and exemplified using the application of membrane protein crystallization within lipidic cubic phases.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Bacteriorhodopsins / chemistry*
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Crystallization
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High-Throughput Screening Assays*
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Humans
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Lipids / chemical synthesis*
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Lipids / chemistry*
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Liquid Crystals / chemistry
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Models, Molecular
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Peptide Library
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Protein Conformation
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Receptors, Dopamine D2 / genetics
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Receptors, Dopamine D2 / isolation & purification
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Receptors, Dopamine D2 / metabolism
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Receptors, Histamine H1 / genetics
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Receptors, Histamine H1 / isolation & purification
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Receptors, Histamine H1 / metabolism
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Reproducibility of Results
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Robotics
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Scattering, Small Angle
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X-Ray Diffraction
Substances
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Lipids
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Peptide Library
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Receptors, Dopamine D2
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Receptors, Histamine H1
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Bacteriorhodopsins