Expression, purification and crystallization of an indole prenyltransferase from Aspergillus fumigatus

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Mar 1;68(Pt 3):355-8. doi: 10.1107/S1744309112004964. Epub 2012 Feb 23.

Abstract

CdpNPT from Aspergillus fumigatus is a dimethylallyltryptophan synthase/indole prenyltransferase that catalyzes reverse prenylation at position N1 of tryptophan-containing cyclic dipeptides. Residues 38-440 of CdpNPT were expressed in Escherichia coli and crystallized using the sitting-drop vapour-diffusion and microseeding techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 84.4, b = 157.1, c = 161.8 Å, α = β = γ = 90.0°.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus fumigatus / enzymology*
  • Crystallization
  • Crystallography, X-Ray
  • Dimethylallyltranstransferase / chemistry*
  • Dimethylallyltranstransferase / genetics
  • Dimethylallyltranstransferase / isolation & purification
  • Gene Expression

Substances

  • Dimethylallyltranstransferase