Chondroitin sulfate and epidermal growth factor immobilization after plasma polymerization: a versatile anti-apoptotic coating to promote healing around stent grafts

Macromol Biosci. 2012 Jun;12(6):812-21. doi: 10.1002/mabi.201100447. Epub 2012 Mar 27.

Abstract

Bioactive coatings constitute an interesting approach to enhance healing around implants, such as stent-grafts used in endovascular aneurysm repair. Three different plasma techniques, namely NH₃ plasma functionalization and atmospheric- or low-pressure plasma polymerization, are compared to create amino groups and covalently bind CS and EGF bioactive molecules on PET. The latter presents the greatest potential. CS + EGF coating is shown to strongly decrease cell apoptosis and cell depletion in serum-free medium, while increasing cell growth compared to unmodified PET. This versatile biomimetic coating holds promise in promoting vascular repair around stent-grafts, where resistance to apoptosis is a key issue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aneurysm / therapy
  • Apoptosis / drug effects*
  • Cells, Cultured
  • Chondroitin Sulfates* / chemistry
  • Chondroitin Sulfates* / pharmacology
  • Coated Materials, Biocompatible* / chemistry
  • Coated Materials, Biocompatible* / pharmacology
  • Epidermal Growth Factor* / chemistry
  • Epidermal Growth Factor* / pharmacology
  • Humans
  • Immobilized Proteins* / chemistry
  • Immobilized Proteins* / pharmacology
  • Stents*

Substances

  • Coated Materials, Biocompatible
  • Immobilized Proteins
  • Epidermal Growth Factor
  • Chondroitin Sulfates